GeneTex
United States (US)

MRPS18A antibody

MRPS18A-antibody-GTX87595-IHC-1_18122102_499.jpg

Cat No. GTX87595

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

MRPS18A Antibody detects endogenous levels of total MRPS18A protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS18A.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitochondrial Ribosomal Protein S18A,Humans18B,Mrp-S18-3,Mrps18-3,S18Bmt,Mrps18A

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to this gene is found on chromosome 3p. Alternative splicing results in multiple transcript variants.[provided by RefSeq]

Database

Research Area

DATA IMAGES
MRPS18A-antibody-GTX87595-IHC-1_18122102_499.jpg

GTX87595 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using MRPS18A Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339