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MRPS18C antibody

Anti-MRPS18C antibody used in IHC (Paraffin sections) (IHC-P). GTX87389
Anti-MRPS18C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87389
Anti-MRPS18C antibody used in Western Blot (WB). GTX87389

Cat. No. GTX87389

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all MRPS18C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS18C (71-120).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CGI134 , MRPS181 , MRPS18C , MRPS18c , S18mtc , mitochondrial ribosomal protein S18C , mrps18c

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 8p, 12p, 15q, and 22q. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPS18C antibody used in IHC (Paraffin sections) (IHC-P). GTX87389

GTX87389 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX87389 MRPS18C antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MRPS18C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87389

GTX87389 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX87389 MRPS18C antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MRPS18C antibody used in Western Blot (WB). GTX87389

GTX87389 WB Image

WB analysis of K562 cell lysates using GTX87389 MRPS18C antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Min S et al. Cells 2022; 11 (13) Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399