*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
MRPS21 Antibody detects endogenous levels of total MRPS21 protein.
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
The antiserum was produced against synthesized peptide derived from human MRPS21.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MRP-S21, Mitochondrial 28S ribosomal protein S21, Mitochondrial28S ribosomal protein S21, S21mt, MRPS21, MRP S21
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq]
GTX87804 IHC-P Image
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using MRPS21 Antibody. The picture on the right is treated with the synthesized peptide.
GTX87804 WB Image
Western blot analysis of extracts from COS cells, using MRPS21 Antibody. The lane on the right is treated with the synthesized peptide.
GTX87804 ICC/IF Image
Immunofluorescence analysis of MCF7 cells, using MRPS21 Antibody. The picture on the right is treated with the synthesized peptide.