APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
| 1:100~1:500 |
| 1:50~1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS21 (38-87).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MDS016 , MRPS21 , RPMS21 , mitochondrial ribosomal protein S21
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX87804 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX87804 MRPS21 antibody. The picture on the right is blocked with the synthesized peptide.
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GTX87804 WB Image
WB analysis of COS cell lysates using GTX87804 MRPS21 antibody. The lane on the right is blocked with the synthesized peptide.
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GTX87804 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX87804 MRPS21 antibody. The picture on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for MRPS21 antibody (GTX87804). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MRPS21 antibody (GTX87804). Be the first to share your experience with this product.
