GeneTex
United States (US)

MRPS21 antibody

MRPS21-antibody-GTX87804-IHC-1_18122102_418.jpg
MRPS21-antibody-GTX87804-WB-1_18121411_290.jpg
MRPS21-antibody-GTX87804-ICC_IF-1_18121411_358.jpg

Cat No. GTX87804

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Specificity/Sensitivity

MRPS21 Antibody detects endogenous levels of total MRPS21 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MRPS21.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MRP-S21, Mitochondrial 28S ribosomal protein S21, Mitochondrial28S ribosomal protein S21, S21mt, MRPS21, MRP S21

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
MRPS21-antibody-GTX87804-IHC-1_18122102_418.jpg

GTX87804 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using MRPS21 Antibody. The picture on the right is treated with the synthesized peptide.

MRPS21-antibody-GTX87804-WB-1_18121411_290.jpg

GTX87804 WB Image

Western blot analysis of extracts from COS cells, using MRPS21 Antibody. The lane on the right is treated with the synthesized peptide.

MRPS21-antibody-GTX87804-ICC_IF-1_18121411_358.jpg

GTX87804 ICC/IF Image

Immunofluorescence analysis of MCF7 cells, using MRPS21 Antibody. The picture on the right is treated with the synthesized peptide.