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MRPS23 antibody

Anti-MRPS23 antibody used in Western Blot (WB). GTX119012
Anti-MRPS23 antibody used in IHC (Paraffin sections) (IHC-P). GTX119012
Anti-MRPS23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119012

Cat. No. GTX119012

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MRPS23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPS23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618108

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S23 , CGI-138 , HSPC329 , MRP-S23

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 7p. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPS23 antibody used in Western Blot (WB). GTX119012

GTX119012 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX119012 diluted at 1:1000

Anti-MRPS23 antibody used in IHC (Paraffin sections) (IHC-P). GTX119012

GTX119012 IHC-P Image

MRPS23 antibody detects MRPS23 protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MRPS23 antibody (GTX119012) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MRPS23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119012

GTX119012 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MRPS23(GTX119012) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169