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MRPS25 antibody [AT38E7]

Anti-MRPS25 antibody [AT38E7] used in Western Blot (WB). GTX53743

Cat No. GTX53743

Host

Mouse

Clonality

Monoclonal

Clone Name

AT38E7

Isotype

IgG2a

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MRPS25 (1-173aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitochondrial Ribosomal Protein S25 , Mrp-S25 , Rpms25 , Mrps25

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPS25 antibody [AT38E7] used in Western Blot (WB). GTX53743

GTX53743 WB Image

WB analysis of HepG2 lysate (40ug) using MRPS25 antibody at the indicated dilutions.

REFERENCE

There are currently no references for MRPS25 antibody [AT38E7] (GTX53743). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf