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MRPS27 antibody

Anti-MRPS27 antibody used in Western Blot (WB). GTX115668
Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115668
Anti-MRPS27 antibody used in IHC (Paraffin sections) (IHC-P). GTX115668

Cat. No. GTX115668

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MRPS27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRPS27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619505

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S27 , MRP-S27 , S27mt

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MRPS27 antibody used in Western Blot (WB). GTX115668

GTX115668 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115668 diluted at 1:1000

Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115668

GTX115668 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MRPS27(GTX115668) antibody at 1:200 dilution.

Anti-MRPS27 antibody used in IHC (Paraffin sections) (IHC-P). GTX115668

GTX115668 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPS27(GTX115668) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169