GeneTex

  • Country / Location Selection

United States (US)

MRPS27 antibody

Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64786
Anti-MRPS27 antibody used in Western Blot (WB). GTX64786
Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64786

Cat. No. GTX64786

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MRPS27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 255-414 of human MRPS27 (NP_055899.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S27 , MRP-S27 , S27mt

Cellular Localization

Cytoplasm,Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64786

GTX64786 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64786 MRPS27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MRPS27 antibody used in Western Blot (WB). GTX64786

GTX64786 WB Image

WB analysis of various sample lysates using GTX64786 MRPS27 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-MRPS27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64786

GTX64786 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64786 MRPS27 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

There are currently no references for MRPS27 antibody (GTX64786). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for MRPS27 antibody (GTX64786). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399