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MRPS28 antibody

Anti-MRPS28 antibody used in Western Blot (WB). GTX66451
Anti-MRPS28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66451
Anti-MRPS28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66451

Cat. No. GTX66451

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MRPS28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-187 of human MRPS28 (NP_054737.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S28 , HSPC007 , MRP-S28 , MRP-S35 , MRPS35

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPS28 antibody used in Western Blot (WB). GTX66451

GTX66451 WB Image

WB analysis of various sample lysates using GTX66451 MRPS28 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:3000
Loading : 25μg per lane

Anti-MRPS28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66451

GTX66451 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX66451 MRPS28 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MRPS28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66451

GTX66451 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66451 MRPS28 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399