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MRPS30 antibody

Anti-MRPS30 antibody used in IHC (Paraffin sections) (IHC-P). GTX54320
Anti-MRPS30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54320
Anti-MRPS30 antibody used in Immunoprecipitation (IP). GTX54320
Anti-MRPS30 antibody used in IHC (Paraffin sections) (IHC-P). GTX54320
Anti-MRPS30 antibody used in Western Blot (WB). GTX54320

Cat. No. GTX54320

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MRPS30 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosomal protein S30 , MRP-S30 , PAP , PDCD9 , S30mt

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MRPS30 antibody used in IHC (Paraffin sections) (IHC-P). GTX54320

GTX54320 IHC-P Image

IHC-P analysis of rat lung tissue using GTX54320 MRPS30 antibody.
Dilution : 1:100

Anti-MRPS30 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54320

GTX54320 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54320 MRPS30 antibody.
Blue : DAPI

Anti-MRPS30 antibody used in Immunoprecipitation (IP). GTX54320

GTX54320 IP Image

IP analysis of HepG2 cell lysate using GTX54320 MRPS30 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-MRPS30 antibody used in IHC (Paraffin sections) (IHC-P). GTX54320

GTX54320 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54320 MRPS30 antibody.
Dilution : 1:100

Anti-MRPS30 antibody used in Western Blot (WB). GTX54320

GTX54320 WB Image

WB analysis of various sample lysates using GTX54320 MRPS30 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Min S et al. Cells 2022; 11 (13) Mitoribosomal Deregulation Drives Senescence via TPP1-Mediated Telomere Deprotection.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399