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MRRF antibody

Anti-MRRF antibody used in Western Blot (WB). GTX117629
Anti-MRRF antibody used in IHC (Paraffin sections) (IHC-P). GTX117629

Cat. No. GTX117629

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MRRF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MRRF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171031

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial ribosome recycling factor , MRFF , MTRRF , RRF

Cellular Localization

Mitochondrion

Database

Research Area

DATA IMAGES

Anti-MRRF antibody used in Western Blot (WB). GTX117629

GTX117629 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
C: HepG2
12% SDS PAGE
GTX117629 diluted at 1:2000

Anti-MRRF antibody used in IHC (Paraffin sections) (IHC-P). GTX117629

GTX117629 IHC-P Image

MRRF antibody detects MRRF protein at cytoplasm in rat skin by immunohistochemical analysis.
Sample: Paraffin-embedded rat skin.
MRRF antibody (GTX117629) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169