PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.
Negative Control
Colon adenocarcinoma with previously documented loss of MSH2 expression: no nuclear staining should be seen in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human MSH2 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mutS homolog 2 , COCA1 , FCC1 , HNPCC , HNPCC1 , LCFS2 , hMSH2
Cellular Localization
Nucleus
Background
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/templates/default/js/lightbox/images/loading.gif "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
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GTX04408 IHC-P Image
IHC-P analysis of human ovarian malignant mixed Müllerian tumor (MMMT) tissue using GTX04408 MSH2 antibody [MSVA-902M] HistoMAX™.
Malignant Mixed Mullerian tumor with strong MSH2 positivity in stromal and inflammatory cells but absence of staining in tumor cells.
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GTX04408 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma (COAD) tissue using GTX04408 MSH2 antibody [MSVA-902M] HistoMAX™.
Colorectal adenocarcinoma with strong MSH2 immunostaining in cancer cells and in stromal cells.
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GTX04408 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04408 MSH2 antibody [MSVA-902M] HistoMAX™.
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REFERENCE
There are currently no references for MSH2 antibody [MSVA-902M] HistoMAX™ (GTX04408). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MSH2 antibody [MSVA-902M] HistoMAX™ (GTX04408). Be the first to share your experience with this product.
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/small/GTX04408/GTX04408_20230728_IHC-P_82_23072722_419.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/small/GTX04408/GTX04408_20230728_IHC-P_212_23072723_420.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/small/GTX04408/GTX04408_20230728_IHC-P_342_23072723_207.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/normal/GTX04408/GTX04408_20230728_IHC-P_82_23072722_419.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/normal/GTX04408/GTX04408_20230728_IHC-P_212_23072723_420.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")
![Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408](/upload/website/prouct_img/normal/GTX04408/GTX04408_20230728_IHC-P_342_23072723_207.webp "Anti-MSH2 antibody [MSVA-902M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04408")