GeneTex
United States (US)

MSH2 antibody

Cat No. GTX16833

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IP, IHC-F
Reactivity Human, Mouse
APPLICATION

Application Note

For IHC-Fr: Use at a concentration of 2 μg/ml. For IHC-P and IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 1.5 μg/ml. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

105 kDa. ( Note )

Positive Control

HCT116 OR SW480 cells
PROPERTIES

Form

Liquid

Buffer

50% Glycerol, 50mM Sodium phosphate, no preservatives added

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant MSH2 protein.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Muts Homolog 2,Coca1,Fcc1,Hnpcc,Hnpcc1,Lcfs2,Hmsh2,Msh2

Cellular Localization

Nuclear

Background

MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world.

Database

Research Area