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MSH3 antibody [C2C3], C-term

Orthogonal
Anti-MSH3 antibody [C2C3], C-term used in Western Blot (WB). GTX113781
Anti-MSH3 antibody [C2C3], C-term used in Western Blot (WB). GTX113781
Anti-MSH3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113781
Anti-MSH3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113781
Anti-MSH3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113781

Cat. No. GTX113781

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MSH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618026

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutS homolog 3 , DUP , FAP4 , MRP1

Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Database

Research Area

DATA IMAGES

Anti-MSH3 antibody [C2C3], C-term used in Western Blot (WB). GTX113781

GTX113781 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (GTX113781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MSH3 antibody [C2C3], C-term used in Western Blot (WB). GTX113781

GTX113781 WB Image

Orthogonal

A549 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (GTX113781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MSH3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113781

GTX113781 IHC-P Image

MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MSH3 stained by MSH3 antibody [C2C3], C-term (GTX113781) diluted at 1:600.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MSH3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113781

GTX113781 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MSH3(GTX113781) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MSH3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113781

GTX113781 ICC/IF Image

MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSH3 stained by MSH3 antibody [C2C3], C-term (GTX113781) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169