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MSH4 antibody

Anti-MSH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX64804
Anti-MSH4 antibody used in Western Blot (WB). GTX64804
Anti-MSH4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64804

Cat. No. GTX64804

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MSH4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 697-936 of human MSH4 (NP_002431.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutS homolog 4

Background

This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-MSH4 antibody used in IHC (Paraffin sections) (IHC-P). GTX64804

GTX64804 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX64804 MSH4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MSH4 antibody used in Western Blot (WB). GTX64804

GTX64804 WB Image

WB analysis of various sample lysates using GTX64804 MSH4 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MSH4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64804

GTX64804 ICC/IF Image

ICC/IF analysis of OVCAR-3 cells using GTX64804 MSH4 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399