APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MSH6 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mutS homolog 6 , GTBP , GTMBP , HNPCC5 , HSAP , p160
Cellular Localization
Nucleus
Background
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530](/templates/default/js/lightbox/images/loading.gif "Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530")
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GTX60530 IHC-P Image
IHC-P analysis of colon cancer tissue using GTX60530 MSH6 antibody [3E1].
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GTX60530 ELISA Image
ELISA analysis of antigen using GTX60530 MSH6 antibody [3E1].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60530 WB Image
WB analysis of HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate using GTX60530 MSH6 antibody [3E1].
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GTX60530 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60530 MSH6 antibody [3E1].
Green : MSH6
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60530 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60530 MSH6 antibody [3E1].
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GTX60530 FACS Image
FACS analysis of MCF-7 cells using GTX60530 MSH6 antibody [3E1].
Green : MSH6
Red : negative control
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GTX60530 WB Image
WB analysis of human MSH6 (AA: 217-395) recombinant protein using GTX60530 MSH6 antibody [3E1].
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REFERENCE
There are currently no references for MSH6 antibody [3E1] (GTX60530). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MSH6 antibody [3E1] (GTX60530). Be the first to share your experience with this product.
![Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_IHC-P_w_23061123_841.webp "Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530")
![Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_ELISA_w_23061123_523.webp "Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530")
![Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_WB_w_23061123_818.webp "Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530")
![Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_ICCIF_w_23061123_722.webp "Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530")
![Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_IHC-P_1_w_23061123_135.webp "Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530")
![Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_FACS_w_23061123_312.webp "Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530")
![Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530](/upload/website/prouct_img/small/GTX60530/GTX60530_20170912_WB_1_w_23061123_784.webp "Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530")
![Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_IHC-P_w_23061123_841.webp "Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530")
![Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_ELISA_w_23061123_523.webp "Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530")
![Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_WB_w_23061123_818.webp "Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530")
![Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_ICCIF_w_23061123_722.webp "Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530")
![Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_IHC-P_1_w_23061123_135.webp "Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530")
![Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_FACS_w_23061123_312.webp "Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530")
![Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530](/upload/website/prouct_img/normal/GTX60530/GTX60530_20170912_WB_1_w_23061123_784.webp "Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530")