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MSH6 antibody [3E1]

Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530
Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530
Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530
Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530
Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530
Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530
Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530

Cat No. GTX60530

Host

Mouse

Clonality

Monoclonal

Clone Name

3E1

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MSH6 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mutS homolog 6 , GTBP , GTMBP , HNPCC5 , HSAP , p160

Cellular Localization

Nucleus

Background

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530

GTX60530 WB Image

WB analysis of HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate using GTX60530 MSH6 antibody [3E1].

Anti-MSH6 antibody [3E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60530

GTX60530 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60530 MSH6 antibody [3E1].
Green : MSH6
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-MSH6 antibody [3E1] used in Western Blot (WB). GTX60530

GTX60530 WB Image

WB analysis of human MSH6 (AA: 217-395) recombinant protein using GTX60530 MSH6 antibody [3E1].

Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530

GTX60530 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60530 MSH6 antibody [3E1].

Anti-MSH6 antibody [3E1] used in ELISA (ELISA). GTX60530

GTX60530 ELISA Image

ELISA analysis of antigen using GTX60530 MSH6 antibody [3E1].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-MSH6 antibody [3E1] used in IHC (Paraffin sections) (IHC-P). GTX60530

GTX60530 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60530 MSH6 antibody [3E1].

Anti-MSH6 antibody [3E1] used in Flow cytometry (FACS). GTX60530

GTX60530 FACS Image

FACS analysis of MCF-7 cells using GTX60530 MSH6 antibody [3E1].
Green : MSH6
Red : negative control

REFERENCE

There are currently no references for MSH6 antibody [3E1] (GTX60530). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MSH6 antibody [3E1] (GTX60530). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339