Wild-type (WT) and MSH6 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH6 antibody [C1C2], Internal (GTX111661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MSH6 antibody [C1C2], Internal detects MSH6 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
5% SDS-PAGE
MSH6 antibody [C1C2], Internal (GTX111661) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using GTBP (MSH6)(GTX111661) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTBP (MSH6)(GTX111661) antibody at 1:500 dilution.

MSH6 antibody [C1C2], Internal immunoprecipitates MSH6 protein in IP experiments.
IP samples: 293T whole cell extract
A. 40 μg 293T whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MSH6 protein by 4 μg MSH6 antibody [C1C2], Internal (GTX111661)
5 % SDS-PAGE
The immunoprecipitated MSH6 protein was detected by MSH6 antibody [C1C2], Internal (GTX111661) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX111661 diluted at 1:1000