MSH6 antibody [C1C2], Internal

Name: MSH6 antibody [C1C2], Internal
Brand: GeneTex
SKU: GTX111661
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-MSH6 antibody [C1C2], Internal used in Western Blot (WB). GTX111661

Anti-MSH6 antibody [C1C2], Internal used in Immunoprecipitation (IP). GTX111661

Anti-MSH6 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111661

Anti-MSH6 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111661

Cat. No. GTX111661

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, EMSA, IHC
Reactivity	Human, Mouse, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all MSH6 products

View product citations for antibody GTX111661 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
EMSA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

Neuro2A , GL261 , HepG2 , HeLa

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MSH6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutS homolog 6 , GTBP , GTMBP , HNPCC5 , HSAP , p160

Cellular Localization

Nucleus

Background

This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer. [provided by RefSeq]

Database

Gene ID: 2956 MSH6
UniProt: P52701 MSH6

Research Area

DATA IMAGES

GTX111661 WB Image

Wild-type (WT) and MSH6 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH6 antibody [C1C2], Internal (GTX111661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111661 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX111661 diluted at 1:1000

GTX111661 IP Image

MSH6 antibody [C1C2], Internal immunoprecipitates MSH6 protein in IP experiments.
IP samples: 293T whole cell extract
A. 40 μg 293T whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MSH6 protein by 4 μg MSH6 antibody [C1C2], Internal (GTX111661)
5 % SDS-PAGE
The immunoprecipitated MSH6 protein was detected by MSH6 antibody [C1C2], Internal (GTX111661) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX111661 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTBP (MSH6)(GTX111661) antibody at 1:500 dilution.

GTX111661 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using GTBP (MSH6)(GTX111661) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111661 WB Image

MSH6 antibody [C1C2], Internal detects MSH6 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
5% SDS-PAGE
MSH6 antibody [C1C2], Internal (GTX111661) dilution: 1:1000

REFERENCE

Application Reference

Fu Y et al. J Immunother Cancer 2023; 11 (4) BRD4 inhibition impairs DNA mismatch repair, induces mismatch repair mutation signatures and creates therapeutic vulnerability to immune checkpoint blockade in MMR-proficient tumors.

Crobach S et al. Fam Cancer 2017; (Epub) Excluding Lynch syndrome in a female patient with metachronous DNA mismatch repair deficient colon- and ovarian cancer.

Application : IHC

Reactivity : Human

Inaguma S et al. Cancer Res 2013; 73 (24) : 7313-23 GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1.

Application : WB

Reactivity : Human

Hsu T et al. Chemosphere 2010; 81 (6) : 748-54 Sublethal levels of cadmium down-regulate the gene expression of DNA mismatch recognition protein MutS homolog 6 (MSH6) in zebrafish (Danio rerio) embryos.

Application : WB

Reactivity : Zebrafish

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