GeneTex
United States (US)

MSL3L1 antibody

GTX105652_40786_IP_19011506_659.jpg
GTX105652_40786_WB_M_Muscle_19011506_378.jpg
GTX105652_40786_WB_19011506_957.jpg
GTX105652_40786_IFA_19011506_534.jpg

Cat No. GTX105652

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , mouse muscle

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MSL3L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp586J1822 antibody, MSL3L1 antibody, MSL3 antibody, MSL3-like 1 antibody, male-specific lethal-3 protein-like 1 antibody, male-specific lethal 3 homolog antibody, male-specific lethal 3 homolog (Drosophila) antibody

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein and has similarity to drosophila male-specific lethal-3 gene. The drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus this encoded protein is thought to play a similar function in chromatin remodeling and transcriptional regulation. This gene has been found to undergo X inactivation. There are four alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq]

Database

DATA IMAGES
GTX105652_40786_IP_19011506_659.jpg

GTX105652 IP Image

MSL3L1 antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 μg of MSL3L1 antibody (GTX105652) 7.5% SDS-PAGE The immunoprecipitated MSL3L1 protein was detected by MSL3L1 antibody (GTX105652) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105652_40786_WB_M_Muscle_19011506_378.jpg

GTX105652 WB Image

Sample (50 ug of whole cell lysate)
A: mouse muscle
10% SDS PAGE
GTX105652 diluted at 1:1000

GTX105652_40786_WB_19011506_957.jpg

GTX105652 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX105652 diluted at 1:500

GTX105652_40786_IFA_19011506_534.jpg

GTX105652 ICC/IF Image

MSL3L1 antibody detects MSL3L1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSL3L1 protein stained by MSL3L1 antibody (GTX105652) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169