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MSL3L1 antibody

Anti-MSL3L1 antibody used in Immunoprecipitation (IP). GTX105652
Anti-MSL3L1 antibody used in Western Blot (WB). GTX105652
Anti-MSL3L1 antibody used in Western Blot (WB). GTX105652
Anti-MSL3L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105652

Cat. No. GTX105652

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MSL3L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

mouse muscle , 293T

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MSL3L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171746

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MSL complex subunit 3 , MSL3L1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein and has similarity to drosophila male-specific lethal-3 gene. The drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus this encoded protein is thought to play a similar function in chromatin remodeling and transcriptional regulation. This gene has been found to undergo X inactivation. There are four alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MSL3L1 antibody used in Immunoprecipitation (IP). GTX105652

GTX105652 IP Image

MSL3L1 antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 μg of MSL3L1 antibody (GTX105652) 7.5% SDS-PAGE The immunoprecipitated MSL3L1 protein was detected by MSL3L1 antibody (GTX105652) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MSL3L1 antibody used in Western Blot (WB). GTX105652

GTX105652 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX105652 diluted at 1:500

Anti-MSL3L1 antibody used in Western Blot (WB). GTX105652

GTX105652 WB Image

Sample (50 ug of whole cell lysate)
A: mouse muscle
10% SDS PAGE
GTX105652 diluted at 1:1000

Anti-MSL3L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105652

GTX105652 ICC/IF Image

MSL3L1 antibody detects MSL3L1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSL3L1 protein stained by MSL3L1 antibody (GTX105652) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169