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MST1 antibody

Anti-MST1 antibody used in Western Blot (WB). GTX103385
Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103385
Anti-MST1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103385

Cat No. GTX103385

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MST1 . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

serine/threonine kinase 4 , KRS2 , MST1 , YSK3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MST1 antibody used in Western Blot (WB). GTX103385

GTX103385 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MST1 antibody (GTX103385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103385

GTX103385 IHC-P Image

STK4 antibody detects STK4 protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
STK4 antibody (GTX103385) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MST1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103385

GTX103385 ICC/IF Image

STK4 antibody detects STK4 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: PC-3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STK4 protein stained by STK4 antibody (GTX103385) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169