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MST1 antibody

KO/KD
Anti-MST1 antibody used in Western Blot (WB). GTX109294
Anti-MST1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109294
Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109294
Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109294
Anti-MST1 antibody used in Immunoprecipitation (IP). GTX109294

Cat. No. GTX109294

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MST1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MST1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 4 , KRS2 , MST1 , YSK3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MST1 antibody used in Western Blot (WB). GTX109294

GTX109294 WB Image

KO/KD

Wild-type (WT) and MST1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MST1 antibody (GTX109294) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MST1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109294

GTX109294 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(GTX109294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109294

GTX109294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(GTX109294) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MST1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109294

GTX109294 IHC-P Image

STK4 antibody detects STK4 protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
STK4 antibody (GTX109294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MST1 antibody used in Immunoprecipitation (IP). GTX109294

GTX109294 IP Image

STK4 antibody immunoprecipitates STK4 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of STK4 protein by 4 μg STK4 antibody (GTX109294)
7.5 % SDS-PAGE
The immunoprecipitated STK4 protein was detected by STK4 antibody (GTX109294) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Scimone C et al. Front Genet 2020; 11 : 146 High-Throughput Sequencing to Detect Novel Likely Gene-Disrupting Variants in Pathogenesis of Sporadic Brain Arteriovenous Malformations.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169