GeneTex
United States (US)

MST3 antibody [N1C1]

GTX107500_39946_IFA_19011506_431.jpg
GTX107500_WB_19011506_529.jpg
GTX107500_39946_IHC_19011506_845.jpg

Cat No. GTX107500

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MST3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MST3 antibody, MST3B antibody, STE20 antibody, STK3 antibody, STK24 antibody, sterile 20-like kinase 3 antibody, serine/threonine-protein kinase 24 antibody, STE20-like kinase 3 antibody, "serine/threonine kinase 24 (STE20 homolog, yeast) antibody", mammalian STE20-like protein kinase 3 antibody, STE20-like kinase MST3 antibody, serine/threonine kinase 24 antibody

Background

The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX107500_39946_IFA_19011506_431.jpg

GTX107500 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using STK24(GTX107500) antibody at 1:100 dilution.

GTX107500_WB_19011506_529.jpg

GTX107500 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX107500 diluted at 1:1000

GTX107500_39946_IHC_19011506_845.jpg

GTX107500 IHC-P Image

STK24 antibody [N1C1] detects STK24 protein at cytosol on HSC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HSC-3 xenograft.
STK24 antibody [N1C1] (GTX107500) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169