APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MST3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952062
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine/threonine kinase 24 , HEL-S-95 , MST3 , MST3B , STE20 , STK3
Background
The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX107500 WB Image
Sample(30μg whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 D: HeLa S3 (GTX14654) E: Hep G2 (GTX27900) F: MOLT4 (GTX27912) G: Raji (GTX27908) 10% SDS PAGE GTX107500 diluted at 1:1000
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GTX107500 IHC-P Image
STK24 antibody [N1C1] detects STK24 protein at cytosol on HSC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HSC-3 xenograft. STK24 antibody [N1C1] (GTX107500) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX107500 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using STK24(GTX107500) antibody at 1:100 dilution.
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REFERENCE
There are currently no references for MST3 antibody [N1C1] (GTX107500). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MST3 antibody [N1C1] (GTX107500). Be the first to share your experience with this product.