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MST3 antibody [N2C1], Internal

Anti-MST3 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113371
Anti-MST3 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113371
Anti-MST3 antibody [N2C1], Internal used in Western Blot (WB). GTX113371
Anti-MST3 antibody [N2C1], Internal used in Western Blot (WB). GTX113371

Cat. No. GTX113371

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MST3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Raji , rat brain

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MST3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952059

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 24 , HEL-S-95 , MST3 , MST3B , STE20 , STK3

Cellular Localization

Cytoplasm

Background

The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MST3 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113371

GTX113371 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STK24(GTX113371) antibody at 1:200 dilution.

Anti-MST3 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113371

GTX113371 IHC-P Image

STK24 antibody [N2C1], Internal detects STK24 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
STK24 antibody [N2C1], Internal (GTX113371) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MST3 antibody [N2C1], Internal used in Western Blot (WB). GTX113371

GTX113371 WB Image

STK24 antibody [N2C1], Internal detects STK24 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
STK24 antibody [N2C1], Internal (GTX113371) dilution: 1:1000

Anti-MST3 antibody [N2C1], Internal used in Western Blot (WB). GTX113371

GTX113371 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX113371 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169