APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1.5μg/ml |
| 2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89947-PEP).
Product Note
This antibody is expected to recognize all three reported isoforms (NP_057626.2, NP_001035918.1 and NP_001035917.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence KLIEKFQKCSADESP, from the C Terminus of the protein sequence according to NP_057626.2; NP_001035918.1; NP_001035917.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine/threonine kinase 26 , MASK , MST4
Cellular Localization
Cytoplasm , Golgi apparatus
Background
The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89947 IHC-P Image
IHC-P analysis of human thymus using GTX89947 MST4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89947 WB Image
WB analysis of Jurkat lysate using GTX89947 MST4 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for MST4 antibody, C-term (GTX89947). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MST4 antibody, C-term (GTX89947). Be the first to share your experience with this product.
