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MST4 antibody [N2C3]

Anti-MST4 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113976
Anti-MST4 antibody [N2C3] used in Western Blot (WB). GTX113976
Anti-MST4 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113976

Cat. No. GTX113976

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MST4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MST4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037908

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 26 , MASK , MST4

Cellular Localization

Cytoplasm , Golgi apparatus

Background

The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MST4 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113976

GTX113976 IHC-P Image

MST4 antibody [N2C3] detects MST4 protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MST4 antibody [N2C3] (GTX113976) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MST4 antibody [N2C3] used in Western Blot (WB). GTX113976

GTX113976 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX113976 diluted at 1:1000

Anti-MST4 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113976

GTX113976 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using MST4 antibody(GTX113976) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169