GeneTex
United States (US)

MTA1 antibody [C1C3]

GTX100610_41073_IFA_19011506_896.jpg
GTX100610_41073_IHC_19011506_751.jpg
GTX100610_41073_IHC_R_19011506_994.jpg
GTX100610_41073_IHC_M_19011506_341.jpg

Cat No. GTX100610

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Metastasis Associated 1,Mta1

Cellular Localization

Isoform Short: Cytoplasm , Isoform Long: Nucleus

Background

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100610_41073_IFA_19011506_896.jpg

GTX100610 ICC/IF Image

MTA1 antibody [C1C3] detects MTA1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MTA1 protein stained by MTA1 antibody [C1C3] (GTX100610) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100610_41073_IHC_19011506_751.jpg

GTX100610 IHC-P Image

MTA1 antibody [C1C3] detects MTA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MTA1 antibody [C1C3] (GTX100610) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100610_41073_IHC_R_19011506_994.jpg

GTX100610 IHC-P Image

MTA1 antibody [C1C3] detects MTA1 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
MTA1 antibody [C1C3] (GTX100610) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100610_41073_IHC_M_19011506_341.jpg

GTX100610 IHC-P Image

MTA1 antibody [C1C3] detects MTA1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
MTA1 antibody [C1C3] (GTX100610) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169