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MTA1 antibody

Anti-MTA1 antibody used in Western Blot (WB). GTX66656

Cat No. GTX66656

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 153-202 of Human MTA1.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

metastasis associated 1

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-MTA1 antibody used in Western Blot (WB). GTX66656

GTX66656 WB Image

WB analysis of various samples using GTX66656 MTA1 antibody.
Lane1 : HEK293T whole cell lysate(10ug)
Lane2 : MCF-7 whole cell lysate(20ug)
Lane3 : HepG2 whole cell lysate(40ug)
Lane4 : H9C2 whole cell lysate(40ug)
Lane5 : CT26 whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349