GeneTex
United States (US)

MTA2 antibody [N1N2], N-term

GTX115203_40254_IHC_18121410_312.jpg
GTX115203_WB_18121410_287.jpg

Cat No. GTX115203

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MTA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Metastasis Associated 1 Family Member 2,Mta1L1,Pid,Mta2

Background

This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115203_40254_IHC_18121410_312.jpg

GTX115203 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MTA2(GTX115203) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115203_WB_18121410_287.jpg

GTX115203 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX115203 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169