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MTAP antibody [N1C3]

Anti-MTAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109117
Anti-MTAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109117
Anti-MTAP antibody [N1C3] used in Immunoprecipitation (IP). GTX109117
Anti-MTAP antibody [N1C3] used in Western Blot (WB). GTX109117
Anti-MTAP antibody [N1C3] used in Western Blot (WB). GTX109117

Cat. No. GTX109117

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MTAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Mouse liver

Predict Reactivity

Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human MTAP Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950953

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylthioadenosine phosphorylase , BDMF , DMSFH , DMSMFH , HEL-249 , LGMBF , MSAP , c86fus

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MTAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109117

GTX109117 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using MTAP(GTX109117) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MTAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109117

GTX109117 ICC/IF Image

MTAP antibody [N1C3] detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MTAP protein stained by MTAP antibody [N1C3] (GTX109117) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MTAP antibody [N1C3] used in Immunoprecipitation (IP). GTX109117

GTX109117 IP Image

Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 μg of MTAP antibody [N1C3] (GTX109117).
Western blot analysis was performed using MTAP antibody [N1C3] (GTX109117).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MTAP antibody [N1C3] used in Western Blot (WB). GTX109117

GTX109117 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX109117 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MTAP antibody [N1C3] used in Western Blot (WB). GTX109117

GTX109117 WB Image

MTAP antibody detects MTAP protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody (GTX109117) diluted at a dilution of 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169