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MTBP Antibody [PHL-1]

Anti-MTBP Antibody [PHL-1] used in Western Blot (WB). GTX48646

Cat No. GTX48646

Host

Mouse

Clonality

Monoclonal

Clone Name

PHL-1

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human, Chimpanzee
Package
50 μl ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:2000
ELISA 1:2000 - 1:20000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MDM2 binding protein , MDM2BP

Background

The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell prolifer-ation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).

Database

Research Area

DATA IMAGES

Anti-MTBP Antibody [PHL-1] used in Western Blot (WB). GTX48646

GTX48646 WB Image

Western blot using GeneTex's anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf