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MTCH2 antibody

KO/KD Orthogonal Overexpression
Anti-MTCH2 antibody used in Western Blot (WB). GTX130324
Anti-MTCH2 antibody used in Western Blot (WB). GTX130324
Anti-MTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130324

Cat. No. GTX130324

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MTCH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

MCF-7 , MTCH2-transfected 293T

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTCH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886241

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial carrier 2 , HSPC032 , MIMP , SLC25A50

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

The substrate transported is not yet known. Induces mitochondrial depolarization.

Database

Research Area

DATA IMAGES

Anti-MTCH2 antibody used in Western Blot (WB). GTX130324

GTX130324 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTCH2 antibody (GTX130324) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MTCH2 antibody used in Western Blot (WB). GTX130324

GTX130324 WB Image

Orthogonal

MTCH2 antibody detects MTCH2 protein by western blot analysis. MCF-7 whole cell extracts and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTCH2 antibody (GTX130324) at a dilution of 1:1000.

Anti-MTCH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130324

GTX130324 IHC-P Image

MTCH2 antibody detects MTCH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MTCH2 antibody (GTX130324) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

MTCH2 antibody Cat. No. GTX130324

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-MTCH2 antibody used in Western Blot (WB). GTX130324
Date : Anonymous submitted on 07-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : T47D cells
Amount used : 20 μg
Blocking : BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production