GeneTex
United States (US)

MTH1 antibody

GTX30108_1327_WB_18121410_735.jpg
GTX30108_1326_WB_18121410_891.jpg
GTX30108_177_ICC-IF_18121410_924.jpg
GTX30108_176_ICC-IF_18121410_106.jpg

Cat No. GTX30108

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
IHC-Fr 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MTH1 antibody

Background

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30108_1327_WB_18121410_735.jpg

GTX30108 WB Image

WB analysis of A431 cell lysate using GTX30108 MTH1 antibody.

GTX30108_1326_WB_18121410_891.jpg

GTX30108 WB Image

WB analysis of (1) NTERA-2, (2) MCF-7-, (3) A431, and (4) COS-7 cell lysate using GTX30108 MTH1 antibody.

GTX30108_177_ICC-IF_18121410_924.jpg

GTX30108 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30108 MTH1 antibody.
Green : primary antibody
Blue : Hoechst 33258

GTX30108_176_ICC-IF_18121410_106.jpg

GTX30108 ICC/IF Image

ICC/IF analysis of A431 cells using GTX30108 MTH1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Package List Price ($)
$ 339