GeneTex
United States (US)

MTHFD2 antibody [N1C3]

KO/KD
GTX104990_40093_20181228_WB_shRNA_watermark_19011519_557.jpg
GTX104990_40093_IHC_19011506_735.jpg
GTX104990_40093_20150420_IP_19011506_165.jpg
GTX104990_40093_20151227_IHC-P_19011506_806.jpg
GTX104990_40093_20151223_IHC-P_19011506_649.jpg
GTX104990_IFA_19011506_317.jpg

Cat No. GTX104990

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , *SK-N-DZ , *SK-N-SH

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NMDMC antibody, MTHFD2 antibody, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase antibody, "bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody", "methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase antibody"

Cellular Localization

Mitochondrion

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX104990_40093_20181228_WB_shRNA_watermark_19011519_557.jpg

GTX104990 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104990_40093_IHC_19011506_735.jpg

GTX104990 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104990_40093_20150420_IP_19011506_165.jpg

GTX104990 IP Image

Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] (GTX104990).
Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104990_40093_20151227_IHC-P_19011506_806.jpg

GTX104990 IHC-P Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human MCF7 xenograft.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104990_40093_20151223_IHC-P_19011506_649.jpg

GTX104990 IHC-P Image

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104990_IFA_19011506_317.jpg

GTX104990 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2(GTX104990) antibody at 1:200 dilution.

Reference
REVIEW

MTHFD2 antibody [N1C3] Cat No. GTX104990

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 04-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : SK-N-DZ cells, SK-N-SH cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Lane Description : Lane1: SK-N-DZ cells Lane2: SK-N-SH cells
SDS
Glycerol.pdf
Thimerosal.pdf