Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MTHFD2 stained by MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MTHFD2 stained by MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] (GTX104990).
Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human MCF7 xenograft.
MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.