GeneTex
United States (US)

MTHFD2 antibody [N3C3]

KO/KD
GTX115482_40457_20181228_WB_shRNA_watermark_19011519_180.jpg
GTX115482_40457_IHC_18121410_853.jpg
GTX115482_40457_IFA_18121410_843.jpg
GTX115482_40457_20190118_WB_R_lung_19022001_541.jpg
GTX115482_40457_20190118_WB_19022001_703.jpg
GTX115482_40457_20190118_WB_M_pancreas_19022001_159.jpg

Cat No. GTX115482

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Methylenetetrahydrofolate Dehydrogenase (Nadp+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase,Nmdmc,Mthfd2

Cellular Localization

Mitochondrion

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115482_40457_20181228_WB_shRNA_watermark_19011519_180.jpg

GTX115482 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX115482_40457_IHC_18121410_853.jpg

GTX115482 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MTHFD2(GTX115482) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115482_40457_IFA_18121410_843.jpg

GTX115482 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MTHFD2(GTX115482) antibody at 1:500 dilution.

GTX115482_40457_20190118_WB_R_lung_19022001_541.jpg

GTX115482 WB Image

Whole cell extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX115482_40457_20190118_WB_19022001_703.jpg

GTX115482 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115482_40457_20190118_WB_M_pancreas_19022001_159.jpg

GTX115482 WB Image

Whole cell extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169