GeneTex

  • Country / Location Selection

United States (US)

MTHFD2 antibody [N3C3]

KO/KD
Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482
Anti-MTHFD2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115482
Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482
Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482
Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482
Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482
Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482
Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482

Cat. No. GTX115482

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MTHFD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse pancreas , rat lung

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719895

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase , NMDMC

Cellular Localization

Mitochondrion

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482

GTX115482 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MTHFD2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115482

GTX115482 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MTHFD2(GTX115482) antibody at 1:500 dilution.

Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482

GTX115482 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MTHFD2(GTX115482) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482

GTX115482 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482

GTX115482 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482

GTX115482 IHC-P Image

MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MTHFD2 antibody [N3C3] used in Western Blot (WB). GTX115482

GTX115482 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MTHFD2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115482

GTX115482 IHC-P Image

MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chan CH et al. Aging (Albany NY) 2020; 12 (18) : 17930-17947 Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer.
Application : WB
Reactivity : Human
Costas Koufaris et al. Cancer & Metabolism 2018; 6 : 12 Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Application : WB
Reactivity : Human
Selcuklu SD et al. J Biol Chem 2012; 287 (35) : 29516-28 MicroRNA-9 inhibition of cell proliferation and identification of novel miR-9 targets by transcriptome profiling in breast cancer cells.
Submit a Reference

REVIEW

There are currently no reviews for MTHFD2 antibody [N3C3] (GTX115482). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169