Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MTHFD2(GTX115482) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using MTHFD2(GTX115482) antibody at 1:500 dilution.