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MTHFD2 antibody

Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519
Anti-MTHFD2 antibody used in Western Blot (WB). GTX64519
Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519
Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519
Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519
Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519
Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519
Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519

Cat. No. GTX64519

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MTHFD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase , NMDMC

Cellular Localization

Mitochondrion

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519

GTX64519 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64519 MTHFD2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MTHFD2 antibody used in Western Blot (WB). GTX64519

GTX64519 WB Image

WB analysis of various sample lysates using GTX64519 MTHFD2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519

GTX64519 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64519 MTHFD2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MTHFD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64519

GTX64519 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64519 MTHFD2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519

GTX64519 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX64519 MTHFD2 antibody.
Dilution : 1:100

Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519

GTX64519 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64519 MTHFD2 antibody.
Dilution : 1:100

Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519

GTX64519 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64519 MTHFD2 antibody.
Dilution : 1:100

Anti-MTHFD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64519

GTX64519 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX64519 MTHFD2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399