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MTHFR antibody [5D3]

Anti-MTHFR antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60435
Anti-MTHFR antibody [5D3] used in Western Blot (WB). GTX60435
Anti-MTHFR antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60435
Anti-MTHFR antibody [5D3] used in ELISA (ELISA). GTX60435
Anti-MTHFR antibody [5D3] used in Western Blot (WB). GTX60435

Cat. No. GTX60435

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D3

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399)
See all MTHFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MTHFR expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887966

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methylenetetrahydrofolate reductase

Background

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-MTHFR antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60435

GTX60435 IHC-P Image

IHC-P analysis of lung cancer using GTX60435 MTHFR antibody [5D3].

Anti-MTHFR antibody [5D3] used in Western Blot (WB). GTX60435

GTX60435 WB Image

WB analysis of HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate using GTX60435 MTHFR antibody [5D3].

Anti-MTHFR antibody [5D3] used in IHC (Paraffin sections) (IHC-P). GTX60435

GTX60435 IHC-P Image

IHC-P analysis of intima cancer tissue (left) and prostate tissue (right) using GTX60435 MTHFR antibody [5D3].

Anti-MTHFR antibody [5D3] used in ELISA (ELISA). GTX60435

GTX60435 ELISA Image

ELISA analysis of antigen using GTX60435 MTHFR antibody [5D3].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-MTHFR antibody [5D3] used in Western Blot (WB). GTX60435

GTX60435 WB Image

WB analysis of Rat heart tissue lysate using GTX60435 MTHFR antibody [5D3].

REFERENCE

Application Reference

Gajjar KK et al. Int J Biol Markers 2018; 33 (2) : 180-188 deciphering the potential value of 5-* metabolic enzymes in predicting prognosis and treatment response of colorectal cancer patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399