Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MTHFR antibody [N3C3] (GTX100535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX100535 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.