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MTMR12 antibody

Anti-MTMR12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119163
Anti-MTMR12 antibody used in Western Blot (WB). GTX119163
Anti-MTMR12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX119163

Cat. No. GTX119163

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MTMR12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MTMR12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618229

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myotubularin related protein 12 , 3-PAP , PIP3AP

Cellular Localization

Cytoplasm

Background

Phosphatidylinositide 3-kinase-derived membrane-anchored phosphatidylinositides, such as phosphatidylinositol 3-phosphate (PtdIns(3)P), regulate diverse cellular processes. The protein encoded by this gene functions as an adaptor subunit in a complex with an active PtdIns(3)P 3-phosphatase. Alternatively spliced transcript variants have been identified, but their biological validity has not been determined. [provided by RefSeq]

Database

DATA IMAGES

Anti-MTMR12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119163

GTX119163 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MTMR12(GTX119163) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MTMR12 antibody used in Western Blot (WB). GTX119163

GTX119163 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX119163 diluted at 1:1000

Anti-MTMR12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX119163

GTX119163 IHC-Fr Image

The data was published in the journal PLoS Genet in 2013. PMID: 23818870

REFERENCE

Application Reference

Sarikaya E et al. Dis Model Mech 2022; 15 (7) Natural history of a mouse model of X-linked myotubular myopathy.
Liu J et al. Oncol Lett 2017; 14 (5) : 5711-5718 miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Gupta VA et al. PLoS Genet 2013; 9 (6) : e1003583 Loss of catalytically inactive lipid phosphatase myotubularin-related protein 12 impairs myotubularin stability and promotes centronuclear myopathy in zebrafish.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169