GeneTex
United States (US)

MUC1 antibody [C595 (NCRC48)]

GTX30742_4997_ELISA_18121410_543.jpg
GTX30742_4996_ELISA_18121410_603.jpg

Cat No. GTX30742

Host Mouse
Clonality Monoclonal
Clone Name C595 (NCRC48)
Isotype IgG3
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA 1/100-1/1,000

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Urinary MUC-1 mucin.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX30742_4997_ELISA_18121410_543.jpg

GTX30742 ELISA Image

Sandwich ELISA analysis of purified breast cancer antigen protein using GTX30742 as a capture reagent and biotinylated Mouse anti Human MUC1, clone VU-4H5 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.

GTX30742_4996_ELISA_18121410_603.jpg

GTX30742 ELISA Image

Sandwich ELISA analysis of purified breast cancer antigen protein using GTX30742 MUC1 antibody as a capture reagent and biotinylated Mouse anti Human MUC1, clone 6A4 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.