MUC1 antibody [HMPV]

Cat No. GTX34851

Host	Mouse
Clonality	Monoclonal
Clone Name	HMPV
Isotype	IgG1
Application	ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($469)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Product Note

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (200kDa) and an amino acid composition rich in serine, threonine, proline and glycine.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human breast cancer cell line ZR-75 cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADMCKD , ADMCKD1 , CA 153 , CD227 , EMA , H23AG , KL6 , MAM6 , MCD , MCKD , MCKD1 , MUC1 , MUC1/SEC , MUC1/X , MUC1/ZD , PEM , PEMT , PUM , mucin 1, cell surface associated , Mucin1 , CA15-3 , CA15.3

Cellular Localization

Apical cell membrane,Secreted,Cell membrane,Cytoplasm,Nucleus

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Gene ID: 4582 MUC1
UniProt: P15941 MUC1

Research Area

DATA IMAGES

GTX34851 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed K562 cells using GTX34851 MUC1 antibody [HMPV].
Green : Primary antibody
Red : nucleus

GTX34851 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX34851 MUC1 antibody [HMPV].
Blue : Primary antibody
Red : Isotype control

GTX34851 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34851 MUC1 antibody [HMPV].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 469

Qty