APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (200kDa) and an amino acid composition rich in serine, threonine, proline and glycine.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human breast cancer cell line ZR-75 cells
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADMCKD , ADMCKD1 , CA 153 , CD227 , EMA , H23AG , KL6 , MAM6 , MCD , MCKD , MCKD1 , MUC1 , MUC1/SEC , MUC1/X , MUC1/ZD , PEM , PEMT , PUM , mucin 1, cell surface associated , Mucin1 , CA15-3 , CA15.3
Cellular Localization
Apical cell membrane,Secreted,Cell membrane,Cytoplasm,Nucleus
Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
![Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851](/templates/default/js/lightbox/images/loading.gif "Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851")
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GTX34851 ICC/IF Image
ICC/IF analysis of paraformaldehyde-fixed K562 cells using GTX34851 MUC1 antibody [HMPV].
Green : Primary antibody
Red : nucleus
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GTX34851 FACS Image
FACS analysis of PFA-fixed MCF-7 cells using GTX34851 MUC1 antibody [HMPV].
Blue : Primary antibody
Red : Isotype control
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GTX34851 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX34851 MUC1 antibody [HMPV].
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REFERENCE
There are currently no references for MUC1 antibody [HMPV] (GTX34851). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MUC1 antibody [HMPV] (GTX34851). Be the first to share your experience with this product.
![Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851](/upload/website/prouct_img/small/GTX34851/GTX34851_20200115_ICC-IF_1663_w_23060801_861.webp "Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851")
![Anti-MUC1 antibody [HMPV] used in Flow cytometry (FACS). GTX34851](/upload/website/prouct_img/small/GTX34851/GTX34851_20200115_FACS_1722_w_23060801_441.webp "Anti-MUC1 antibody [HMPV] used in Flow cytometry (FACS). GTX34851")
![Anti-MUC1 antibody [HMPV] used in IHC (Paraffin sections) (IHC-P). GTX34851](/upload/website/prouct_img/small/GTX34851/GTX34851_20200115_IHC-P_824_w_23060801_425.webp "Anti-MUC1 antibody [HMPV] used in IHC (Paraffin sections) (IHC-P). GTX34851")
![Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851](/upload/website/prouct_img/normal/GTX34851/GTX34851_20200115_ICC-IF_1663_w_23060801_861.webp "Anti-MUC1 antibody [HMPV] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34851")
![Anti-MUC1 antibody [HMPV] used in Flow cytometry (FACS). GTX34851](/upload/website/prouct_img/normal/GTX34851/GTX34851_20200115_FACS_1722_w_23060801_441.webp "Anti-MUC1 antibody [HMPV] used in Flow cytometry (FACS). GTX34851")
![Anti-MUC1 antibody [HMPV] used in IHC (Paraffin sections) (IHC-P). GTX34851](/upload/website/prouct_img/normal/GTX34851/GTX34851_20200115_IHC-P_824_w_23060801_425.webp "Anti-MUC1 antibody [HMPV] used in IHC (Paraffin sections) (IHC-P). GTX34851")