GeneTex
United States (US)

MUC1 antibody [M10H6]

Cat No. GTX10117

Host Mouse
Clonality Monoclonal
Clone Name M10H6
Isotype IgG
Application ICC/IF, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

25.41 kDa. ( Note )

Specificity/Sensitivity

This products reacts with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosilated MUC1 prepared from tumor fluids or as a result of chemical treatment of the human milk MUC1. This product does not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs and belongs to Mab cluster 1-4 according to ISOMB TD-4 classification.No cross-reactivity with egg white avidin. Specific to unglycosylated VNTR region.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

High molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Research Area