Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
ELISA
This antibody can be used for detection of glycosylated tumor-associated MUC1 mucins in human serum.
Sandwich ELISA
Capture : GTX28322/GTX10120/GTX10123, Dection: GTX10118 (detection of glycosylated MUC1)
|
Not tested in other applications.
Calculated MW
Product Note
Clone M4H2, M2F1, M2C5 bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions. Clone M2F1 and M2C5 efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. Belong to monoclonal antibody clusters 6 and 7 according to ISOMB TD-4 classification. No cross reactive with normal serum proteins and human tissues of non-epithelial origin.
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
High molecular weight (>300 kDa) glycoprotein from human milk-fat globule molecule. This antibody binds with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_368630
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM
Cellular Localization
Apical cell membrane , Cytoplasm , Nucleus
Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Database
Research Area
