*Optimal dilutions/concentrations should be determined by the researcher.
This antibody can be used for detection of unglycosylated and glycosylated tumor-associated MUC1 mucins in human serum.
Capture : GTX10120, Dection: GTX10120 (detection of unglycosylated MUC1) or Capture : GTX10120, Dection: GTX10118/GTX28323 (detection of glycosylated MUC1)
Not tested in other applications.
Clone M4H2, M2F1, M2C5 bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions. Clone M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein, with monomeric VNTR MUC1 peptide, with deglycosylated and underglycosylated natural MUC1 protein.
PBS pH 7.4, 0.09 % sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.
Batch dependent (Please refer to the vial label for the specific concentration.)
Recombinant (E. coli) VNTR20 (tandem repeat in MUC1 core) polypeptide. This antibody binds with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mucin 1, Cell Surface Associated , Admckd , Admckd1 , Ca 15-3 , Cd227 , Ema , H23Ag , Kl-6 , Mam6 , Mcd , Mckd , Mckd1 , Muc-1 , Muc-1/Sec , Muc-1/X , Muc1/Zd , Pem , Pemt , Pum , Muc1
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]