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MUC1 antibody [M4H2]

Cat No. GTX10120

Host

Mouse

Clonality

Monoclonal

Clone Name

M4H2

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, ELISA, sELISA

Reactivity

Human
Package
100 μg ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
sELISA Assay dependent

Note :

ELISA
This antibody can be used for detection of unglycosylated and glycosylated tumor-associated MUC1 mucins in human serum.

sELISA
Capture : GTX10120, Dection: GTX10120 (detection of unglycosylated MUC1) or Capture : GTX10120, Dection: GTX10118/GTX28323 (detection of glycosylated MUC1)

Not tested in other applications.

Calculated MW

25.41 kDa. ( Note )

Specificity/Sensitivity

Clone M4H2, M2F1, M2C5 bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions. Clone M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein, with monomeric VNTR MUC1 peptide, with deglycosylated and underglycosylated natural MUC1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant (E. coli) VNTR20 (tandem repeat in MUC1 core) polypeptide. This antibody binds with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide-carbohydrate compositions.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mucin 1, Cell Surface Associated , Admckd , Admckd1 , Ca 15-3 , Cd227 , Ema , H23Ag , Kl-6 , Mam6 , Mcd , Mckd , Mckd1 , Muc-1 , Muc-1/Sec , Muc-1/X , Muc1/Zd , Pem , Pemt , Pum , Muc1

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 219