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MUC1 antibody [MUC1/955]

Anti-MUC1 antibody [MUC1/955] used in Western Blot (WB). GTX34852
Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852
Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852
Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852

Cat No. GTX34852

Host

Mouse

Clonality

Monoclonal

Clone Name

MUC1/955

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
ELISA 2-5μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human milk-fat globule membranes (HMFGM)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADMCKD , ADMCKD1 , CA 153 , CD227 , EMA , H23AG , KL6 , MAM6 , MCD , MCKD , MCKD1 , MUC1 , MUC1/SEC , MUC1/X , MUC1/ZD , PEM , PEMT , PUM , mucin 1, cell surface associated , Mucin1 , CA15-3 , CA15.3

Cellular Localization

Apical cell membrane,Secreted,Cell membrane,Cytoplasm,Nucleus

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-MUC1 antibody [MUC1/955] used in Western Blot (WB). GTX34852

GTX34852 WB Image

WB analysis of T47D cell lysate using GTX34852 MUC1 antibody [MUC1/955].

Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852

GTX34852 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34852 MUC1 antibody [MUC1/955].

Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852

GTX34852 IHC-P Image

IHC-P analysis of human endometrial carcinoma tissue using GTX34852 MUC1 antibody [MUC1/955].

Anti-MUC1 antibody [MUC1/955] used in IHC (Paraffin sections) (IHC-P). GTX34852

GTX34852 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34852 MUC1 antibody [MUC1/955].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469