United States (US)

MUC1 antibody [SPM132]

Anti-MUC1 antibody [SPM132] used in IHC (Paraffin sections) (IHC-P). GTX34856

Cat No. GTX34856

Host Mouse
Clonality Monoclonal
Clone Name SPM132
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.1-0.2ug/ml for 30 minutes at RT

Note :

Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

122 kDa. ( Note )


In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The β subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.




Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



Human milk fat globule membranes


Ab purified from Bioreactor Concentrate by Protein A/G




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1


This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]


Research Area

Anti-MUC1 antibody [SPM132] used in IHC (Paraffin sections) (IHC-P). GTX34856

GTX34856 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Cancer stained with EMA Monoclonal Antibody (SPM132).