GeneTex
United States (US)

MUC1 antibody

GTX100459_42529_20160728_WB_19011506_290.jpg
GTX100459_40268_20150420_IHC_19011506_405.jpg
GTX100459_40268_IP_19011506_608.jpg
GTX100459_40268_20160713_IFA_19011506_106.jpg
GTX100459_40268_IFA_19011506_834.jpg

Cat No. GTX100459

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

MCF-7
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MUC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1

Cellular Localization

Apical cell membrane; Single-pass type I membrane protein , Isoform 5: Secreted , Isoform 7: Secreted , Isoform 9: Secreted , Mucin-1 subunit beta: Cell membrane , Cytoplasm , Nucleus

Background

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100459_42529_20160728_WB_19011506_290.jpg

GTX100459 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC1 antibody (GTX100459) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 21927028

GTX100459_40268_20150420_IHC_19011506_405.jpg

GTX100459 IHC-P Image

MUC1 antibody detects MUC1 protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
MUC1 antibody (GTX100459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100459_40268_IP_19011506_608.jpg

GTX100459 IP Image

Immunoprecipitation of MUC1 protein from HCT-116 whole cell extracts using 5 μg of MUC1 antibody (GTX100459).
Western blot analysis was performed using MUC1 antibody (GTX100459).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100459_40268_20160713_IFA_19011506_106.jpg

GTX100459 ICC/IF Image

MUC1 antibody detects MUC1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: MUC1 protein stained by MUC1 antibody (GTX100459) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100459_40268_IFA_19011506_834.jpg

GTX100459 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUC1(GTX100459) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf