GeneTex
United States (US)

MUC1 antibody

MUC1-antibody_IHC-P_GTX15481-1_18121411_247.jpg

Cat No. GTX15481

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

Recommended Starting Dilutions:
For ICC/IF: Use at a dilution of 1:500
For IHC-P: Use at a dilution of 1:100. No special pretreatment is required for immunohistochemistry.
For IP: Use at an assay dependent dilutions.
For WB: Use at an assay dependent dilution.
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

122 kDa. ( Note )

Positive Control

MCF-7 cells and Breast carcinoma
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acid 1067 and 1255 of human MUC1 (Swiss-Prot entry P15941).

Purification

Immunogen affinity purified
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mucin 1, Cell Surface Associated,Admckd,Admckd1,Ca 15-3,Cd227,Ema,H23Ag,Kl-6,Mam6,Mcd,Mckd,Mckd1,Muc-1,Muc-1/Sec,Muc-1/X,Muc1/Zd,Pem,Pemt,Pum,Muc1

Cellular Localization

Cell Membrane and Cytoplasmic

Background

MUC-1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

Database

Research Area

DATA IMAGES
MUC1-antibody_IHC-P_GTX15481-1_18121411_247.jpg

GTX15481 IHC-P Image

Human Breast Carcinoma stained with anti-MUC-1/EMA/Epithelial Membrane Antigen antibody (GTX15481)

REFERENCE