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MUC2 antibody [C3], C-term

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

Cat No. GTX100664

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

540 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mucin 2, oligomeric mucus/gel-forming , MLP , MUC-2 , SMUC

Cellular Localization

Secreted

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein in human intestine by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
MUC2 antibody [C3], C-term (GTX100664) diluted at 1:750.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

MUC2 antibody [C3], C-term Cat No. GTX100664

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 01-Nov-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : LS174T cells
Blocking : 2% BSA in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 2% Triton-X100 in PBS
Primary Antibody : 1:100, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169