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MUC2 antibody [C3], C-term

Orthogonal
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

Cat No. GTX100664

Reference 15
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all MUC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

540 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mucin 2, oligomeric mucus/gel-forming , MLP , MUC-2 , SMUC

Cellular Localization

Secreted

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein in human intestine by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
MUC2 antibody [C3], C-term (GTX100664) diluted at 1:750.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wang S et al. Theranostics 2020; 10 (22) : 10171-10185 Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
Application : IHC-P
Reactivity : Mouse
Li C et al. Res Vet Sci 2020; 133 : 75-84 Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine.
Application : IHC-P
Reactivity : Mouse
van der Hee B et al. Front Cell Dev Biol 2020; 8 : 375 Congruence of Transcription Programs in Adult Stem Cell-Derived Jejunum Organoids and Original Tissue During Long-Term Culture.
Application : IHC-P
Ma S et al. Sci Rep 2020; 10 (1) : 5523 Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model.
Application : ICC/IF
Reactivity : Human
Yeom J et al. Antioxidants (Basel) 2020; 9 (3) Oxyresveratrol Induces Autophagy via the ER Stress Signaling Pathway, and Oxyresveratrol-Induced Autophagy Stimulates MUC2 Synthesis in Human Goblet Cells.
Application : ICC/IF
Reactivity : Human
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Submit a Reference

REVIEW

MUC2 antibody [C3], C-term Cat No. GTX100664

Rating
( Average 4.5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.5 based on 2 users reviews)
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Antioxidants (Basel) in 2020.PMID: 32150901
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Date : Anonymous submitted on 01-Nov-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : LS174T cells
Blocking : 2% BSA in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 2% Triton-X100 in PBS
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
Application Reference
Wang S et al. Theranostics 2020; 10 (22):10171-10185 Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
Application : IHC-P
Reactivity : Mouse
Li C et al. Res Vet Sci 2020; 133:75-84 Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine.
Application : IHC-P
Reactivity : Mouse
van der Hee B et al. Front Cell Dev Biol 2020; 8:375 Congruence of Transcription Programs in Adult Stem Cell-Derived Jejunum Organoids and Original Tissue During Long-Term Culture.
Application : IHC-P
Ma S et al. Sci Rep 2020; 10 (1):5523 Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model.
Application : ICC/IF
Reactivity : Human
Yeom J et al. Antioxidants (Basel) 2020; 9 (3) Oxyresveratrol Induces Autophagy via the ER Stress Signaling Pathway, and Oxyresveratrol-Induced Autophagy Stimulates MUC2 Synthesis in Human Goblet Cells.
Application : ICC/IF
Reactivity : Human
Song S et al. Biochem Biophys Res Commun 2020; 521 (4):1070-1076 Somatostatin stimulates colonic MUC2 expression through SSTR5-Notch-Hes1 signaling pathway.
Application : ICC/IF
Reactivity : Mouse
Toubai T et al. Nat Microbiol 2019; 4 (5):800-812 Host NLRP6 exacerbates graft-versus-host disease independent of gut microbial composition.
Hwang D et al. Food Chem Toxicol 2018; 118:880-888 Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells.
Application : ICC/IF
Reactivity : Human
Ozcan HEA et al. Adv Med Sci 2018; 63 (2):296-300 Expression profile and cellular localizations of mucin proteins, CK7, and cytoplasmic p27 in Barrett's esophagus and esophageal adenocarcinoma.
Application : IHC-P
Reactivity : Human
Jinfang et al. Digestive and Liver Disease 2018; (Epub) Peroxisome Proliferator-activated Receptor Gamma Activation Promotes Intestinal Barrier Function by Improving Mucus and Tight Junctions in a Mouse Colitis Model.
Application : IHC-P
Reactivity : Mouse
Sun C et al. Inflamm Bowel Dis 2017; 23 (8):1338-1347 Cideb Deficiency Aggravates Dextran Sulfate Sodium-induced Ulcerative Colitis in Mice by Exacerbating the Oxidative Burden in Colonic Mucosa.
Application : IHC-P
Reactivity : Mouse
Hwang D et al. J Funct Foods 2017; 35:146-158 Oxyresveratrol-containing Ramulus mori ethanol extract attenuates acute colitis by suppressing inflammation and increasing mucin secretion.
Application : ICC/IF
Reactivity : Human
Usui T et al. Stem Cells International 2016; 2016:Article ID 7053872 Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment.
Application : ICC/IF
Reactivity : Human
Wang K et al. Immunity 2014; 41 (6):1052-63 Interleukin-17 receptor a signaling in transformed enterocytes promotes early colorectal tumorigenesis.
Application : IHC-Fr
Reactivity : Mouse
Grivennikov SI et al. Nature 2012; 491 (7423):254-8 Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
Application : IHC
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169