GeneTex
United States (US)

MUC2 antibody [C3], C-term

GTX100664_43041_20171121_IHC-P_M_1_19011506_977.jpg
GTX100664_43041_20171121_IHC-P_M_19011506_437.jpg
GTX100664_42774_20170926_IHC-P_1_19011506_398.jpg

Cat No. GTX100664

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

540 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MLP antibody, MUC-2 antibody, SMUC antibody, MUC2 antibody, "mucin 2, intestinal/tracheal antibody", mucin-like protein antibody, intestinal mucin-2 antibody, mucin-2 antibody, "mucin 2, oligomeric mucus/gel-forming antibody"

Cellular Localization

Secreted

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100664_43041_20171121_IHC-P_M_1_19011506_977.jpg

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100664_43041_20171121_IHC-P_M_19011506_437.jpg

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100664_42774_20170926_IHC-P_1_19011506_398.jpg

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein in human intestine by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
MUC2 antibody [C3], C-term (GTX100664) diluted at 1:750.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf