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MUC2 antibody [HL1724]

Recombinant antibody
Anti-MUC2 antibody [HL1724] used in Western Blot (WB). GTX637372
Anti-MUC2 antibody [HL1724] used in IHC (Paraffin sections) (IHC-P). GTX637372
Anti-MUC2 antibody [HL1724] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637372
Anti-MUC2 antibody [HL1724] used in IHC (Paraffin sections) (IHC-P). GTX637372

Cat. No. GTX637372

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1724

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MUC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

540 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human MUC2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mucin 2, oligomeric mucus/gel-forming , MLP , MUC-2 , SMUC

Cellular Localization

Secreted

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-MUC2 antibody [HL1724] used in Western Blot (WB). GTX637372

GTX637372 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC2 antibody [HL1724] (GTX637372) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MUC2 antibody [HL1724] used in IHC (Paraffin sections) (IHC-P). GTX637372

GTX637372 IHC-P Image

MUC2 antibody [HL1724] detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [HL1724] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637372

GTX637372 ICC/IF Image

MUC2 antibody [HL1724] detects MUC2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-MUC2 antibody [HL1724] used in IHC (Paraffin sections) (IHC-P). GTX637372

GTX637372 IHC-P Image

MUC2 antibody [HL1724] detects MUC2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations