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MUC2 antibody [SPM296]

Anti-MUC2 antibody [SPM296] used in IHC (Paraffin sections) (IHC-P). GTX34865

Cat. No. GTX34865

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM296

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all MUC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

540 kDa. ( Note )

Product Note

Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MLP , MUC2 , SMUC , mucin 2, oligomeric mucus/gelforming , mucin 2 , mucin 2, oligomeric mucus/gel-forming

Cellular Localization

Secreted

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-MUC2 antibody [SPM296] used in IHC (Paraffin sections) (IHC-P). GTX34865

GTX34865 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34865 MUC2 antibody [SPM296].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529