IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM citrate buffer for 10 minutes) enhances the signal and may require further dilution of antibody.
Human colon carcinoma, LS 174 T colon cancer cell line
10mM PO4, 200mM NaCl containing 1% BSA and 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
2 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic MUC2 (23 aa) tandem repeat consensus sequence
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mucin 2, Oligomeric Mucus/Gel-Forming , Mlp , Muc-2 , Smuc , Muc2
Cytoplasmic, perinuclear staining of goblet cells.
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]