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MUC2 antibody

Cat No. GTX72840

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC

Reactivity

Human
Package
500 μl ($219)

APPLICATION

Application Note

IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM citrate buffer for 10 minutes) enhances the signal and may require further dilution of antibody.

Calculated MW

540 kDa. ( Note )

Positive Control

Human colon carcinoma, LS 174 T colon cancer cell line

PROPERTIES

Form

Liquid

Buffer

10mM PO4, 200mM NaCl containing 1% BSA and 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic MUC2 (23 aa) tandem repeat consensus sequence

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mucin 2, Oligomeric Mucus/Gel-Forming , Mlp , Muc-2 , Smuc , Muc2

Cellular Localization

Cytoplasmic, perinuclear staining of goblet cells.

Background

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 219