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MURF1 antibody

Overexpression
Anti-MURF1 antibody used in Western Blot (WB). GTX33334
Anti-MURF1 antibody used in Western Blot (WB). GTX33334
Anti-MURF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33334

Cat. No. GTX33334

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MURF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human TRIM63

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 63 , IRF , MURF1 , MURF2 , RNF28 , SMRZ

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-MURF1 antibody used in Western Blot (WB). GTX33334

GTX33334 WB Image

WB analysis of rat heart tissue lysate using GTX33334 MURF1 antibody.
Dilution : 1:1000
Loading : 25μg

Anti-MURF1 antibody used in Western Blot (WB). GTX33334

GTX33334 WB Image

Overexpression

WB analysis of 293F whole cell lysate with/without human TRIM63 protein overexpression using GTX33334 MURF1 antibody.
Dilution : 1:1000
Loading : 25μg

Anti-MURF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33334

GTX33334 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33334 MURF1 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

REFERENCE

Application Reference

Kim Y. et al. Int. J. Environ. Res. Public Health 2021; 18 (5) : 2367 BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function.
Application : WB
Reactivity : Mouse
Lee SJ et al. J Ethnopharmacol 2019; 242 : 112054 Ginsenoside Rg3 upregulates myotube formation and mitochondrial function, thereby protecting myotube atrophy induced by tumor necrosis factor-alpha.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399